GCGR Total GPCR Internalization Assay Kit

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Features Specifications: GCGR Total GPCR Internalization Assay Kit

 Total GPCR Internalization cells are engineered to co-the Enzyme Acceptor (EA) tagged GPCR, and a ProLink (PK) tag localized to the endosomes. Activation of the GPCR-EA induces internalization of the receptor in PK-tagged endosomes, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

 

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GCGR Total GPCR Internalization Assay Kit

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